Coding
Part:BBa_K4011006:Design
Designed by: Aaron Zhexuan Zhang Group: iGEM21_LINKS_China (2021-10-21)
AeAT9
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 199
Illegal BglII site found at 817 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 226
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 1064
Design Notes
Sequence acquired from Shi et al, 2021 (https://doi.org/10.1021/acssynbio.0c00446)
Source
AeAT9 is from Actinidia eriantha.